Disruption of the human gut microbiota affected by ulcerative colitis

  • Haichao Wang School of Biology and Food Engineering, Suzhou University, Suzhou, Anhui, China
  • Changhao Wu School of Biology and Food Engineering, Suzhou University, Suzhou, Anhui, China
  • Dehui Kong School of Biology and Food Engineering, Suzhou University, Suzhou, Anhui, China
Keywords: ulcerative colitis, intestinal flora, high-throughput 16S rRNA sequencing


Objective: The stability of the intestinal flora is an important guarantee for maintaining human health. Whether it will disturb the stability of the flora after ulcerative colitis (UC) is worth exploring.

Methods: An observational study was performed to evaluate the disruption of the human gut microbiota affected by UC. Chromosomal DNA was extracted, and the length of DNA fragments was examined and quantified. Gene amplification and sequencing of the V3 region of 16S rRNA were subsequently performed by Illumina’s MiSeq sequencer for high-throughput sequencing.

Results: The structure of intestinal flora in UC patients exhibited a significant increase of Proteobacteria and a remarkable decrease of Bacteroidetes compared with that of healthy controls. Among each genus in the Proteobacteria, UC patients exhibited a significant decrease of Alcaligenaceae (55.08% ± 13.90 to 0.56% ± 0.04) and a remarkable increase of Enterobacteriaceae (6.64% ± 10.22 to 98.05% ± 3.28) compared with that of healthy controls. Our results demonstrated that the disorder of the intestinal flora is associated with UC patients.

Conclusions: Therefore, UC patients exhibited a significant shift in the dominance of the gut microbiota, and restoring the homeostasis of the intestinal flora may be an important target for the treatment of UC and alleviation of symptoms.


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Ng SC, Tang W, Ching JY, Wong M, Chow CM, Hui AJ, et al. Incidence and phenotype of inflammatory bowel disease based on results from the Asia-pacific Crohn’s and colitis epidemiology study. Gastroenterology 2013; 145(1): 158–65.e2. doi: 10.1053/j.gastro.2013.04.007

Kappelman MD, Moore KR, Allen JK, Cook SF. Recent trends in the prevalence of Crohn’s disease and ulcerative colitis in a commercially insured US population. Dig Dis Sci 2013; 58(2): 519–25. doi: 10.1007/s10620-012-2371-5

Wang YF, Ouyang Q, Hu RW. Progression of inflammatory bowel disease in China. J Dig Dis 2010; 11(2): 76–82. doi: 10.1111/j.1751-2980.2010.00421.x

Fumery M, Singh S, Dulai PS, Gower-Rousseau C, Peyrin-Biroulet L, Sandborn WJ. Natural history of adult ulcerative colitis in population-based cohorts: a systematic review. Clin Gastroenterol Hepatol 2018; 16(3): 343–56.e3. doi: 10.1016/j.cgh.2017.06.016

Mudireddy PR, Blonski W, Lichtenstein GR. The history of medical therapy of ulcerative colitis. In: Lichtenstein GR, ed. Medical therapy of ulcerative colitis. New York, NY: Springer New York; 2014, pp. 1–14.

Abdalla M, Landerholm K, Andersson P, Andersson RE, Myrelid P. Risk of rectal cancer after colectomy for patients with ulcerative colitis: a national cohort study. Clin Gastroenterol Hepatol 2017; 15(7): 1055–60.e2. doi: 10.1016/j.cgh.2016.11.036

Keller DS, Windsor A, Cohen R, Chand M. Colorectal cancer in inflammatory bowel disease: review of the evidence. Tech Coloproctol 2019; 23(1): 3–13. doi: 10.1007/s10151-019-1926-2

Khalili H, Higuchi LM, Ananthakrishnan AN, Manson JE, Feskanich D, Richter JM, et al. Hormone therapy increases risk of ulcerative colitis but not Crohn’s disease. Gastroenterology 2012; 143(5): 1199–206. doi: 10.1053/j.gastro.2012.07.096

Gill SR, Pop M, Deboy RT, Eckburg PB, Turnbaugh PJ, Samuel BS, et al. Metagenomic analysis of the human distal gut microbiome. Science 2006; 312(5778): 1355–9. doi: 10.1126/science.1124234

von Elm E, Altman DG, Egger M, Pocock SJ, Gotzsche PC, Vandenbroucke JP, et al. The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement: guidelines for reporting observational studies. Int J Surg 2014; 12(12): 1495–9. doi: 10.1016/j.ijsu.2014.07.013

Huang G, Ye L, Du G, Huang Y, Wu Y, Ge S, et al. Effects of curcumin plus Soy oligosaccharides on intestinal flora of rats with ulcerative colitis. Cell Mol Biol (Noisy-le-grand) 2017; 63(7): 20–5. doi: 10.14715/cmb/2017.63.7.3

Monif GRG. Is ulcerative colitis a disease of a dysfunctional microbiota? Med Hypotheses 2019; 131: 109300. doi: 10.1016/j.mehy.2019.109300

Shen ZH, Zhu CX, Quan YS, Yang ZY, Wu S, Luo WW, et al. Relationship between intestinal microbiota and ulcerative colitis: mechanisms and clinical application of probiotics and fecal microbiota transplantation. World J Gastroenterol 2018; 24(1): 5–14. doi: 10.3748/wjg.v24.i1.5

How to Cite
WangH., WuC., & KongD. (2022). Disruption of the human gut microbiota affected by ulcerative colitis. STEMedicine, 3(2), e121. https://doi.org/10.37175/stemedicine.v3i2.121
Research articles