High intensity focused ultrasound enhances anti-tumor immunity through promoting CD4 Th1 effector T cell response

  • Haiying Zhang Department of Renal Rheumatism, Shanghai Jiaotong University Affiliated Sixth People’s Hospital East Campus, NO.222 Huanhu West 3rd Road, Nanhui New Town, Pudong New District, Shanghai 201306, China
  • Kun Han Department of Renal Rheumatism, Shanghai Jiaotong University Affiliated Sixth People’s Hospital East Campus, NO.222 Huanhu West 3rd Road, Nanhui New Town, Pudong New District, Shanghai 201306, China
Keywords: Melanoma, HIFU, Anti-tumor immunity, TGFβ, MicroRNA-9-5p

Abstract

Background: Melanoma accounts for more than 80% of deaths from all dermatologic cancers, mainly due to its widespread metastasis. High intensity focused ultrasound (HIFU) is a promising technique for cancer therapy. Here, we investigated the efficacy of HIFU against melanoma and the underlying mechanisms.

Methods: A melanoma allograft mouse model was established to examine the tumor progression and survival rate. Anti-tumor immunity was determined by measuring cytokines, regulatory T cells (Tregs), Th17 cells and CD8+ effector T cells. Western blot, qPCR, RNAi and luciferase assay were performed to confirm the expression and regulation of microRNA (miR)-9-5p and transforming growth factor beta (TGF-β).

Results: HIFU exposure significantly suppressed melanoma growth and metastasis by activating interferon gamma (IFN-γ) secretion, inhibiting Tregs and Th17 cells, and stimulating CD8+ effector T cells. TGF-β was a direct target of miR-9-5p. The anti-tumor effect of HIFU might be mediated through the miR-9-5p/TGF-β pathway.

Conclusion: HIFU activates anti-tumor response and alters tumor microenvironment, which may serve as a potential therapeutic strategy for melanoma treatment.

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Published
2020-09-29
How to Cite
Zhang, H., & Han, K. (2020). High intensity focused ultrasound enhances anti-tumor immunity through promoting CD4 Th1 effector T cell response. STEMedicine, 1(4), e65. https://doi.org/10.37175/stemedicine.v1i4.65
Section
Research articles