RNA-seq analysis reveals that oleic acid exerts anti-inflammatory effects via modulating cell cycle in Raw264.7 cells

  • Taoyu Li School of Pharmaceutical Sciences, South-Central University for Nationalities, No.182, Minzu Avenue, Wuhan, 430074, China.
  • Wanli Liang Bioland Laboratory, (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, 510700, China.
  • Xiaojun Li School of Pharmaceutical Sciences, South-Central University for Nationalities, No.182, Minzu Avenue, Wuhan, 430074, China
  • Wei Kevin Zhang School of Pharmaceutical Sciences, South-Central University for Nationalities, No.182, Minzu Avenue, Wuhan, 430074, China; Bioland Laboratory, (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, 510700, China.
Keywords: Oleic acid, Lipopolysaccharide, Cell cycle control, p21Cip1/Waf1, Transcriptome

Abstract

Background: Lipopolysaccharide (LPS), a structural and protective compound primarily found in ordinary benign bacteria, could induce pro-inflammatory effect in a macrophage cell line Raw264.7 cells. Additionally, we previously showed that oleic acid (OA) possessed apoptotic effect in mouseoriginated Raw264.7 cells as well.

Methods: Cellular and molecular methods including flowcytometry and Western blot as well as bioinformatic methods including RNAseq and analysis.

Results: We demonstrated that OA could alleviate LPS-activated inflammatory effects, including apoptosis and secretion of cytokines via the modulation of cell cycle process. Further analysis revealed that OA reduced the LPS-elevated expression of p21, but not p16.

Conclusion: Our investigation has provided detailed information on LPS stimulation and OA remission in Raw264.7 cells, and laid solid foundation for the potential pharmaceutical application of OA as an antiinflammatory agent.

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Published
2021-03-08
How to Cite
Li, T., Liang, W., Li, X., & Zhang, W. K. (2021). RNA-seq analysis reveals that oleic acid exerts anti-inflammatory effects via modulating cell cycle in Raw264.7 cells. STEMedicine, 2(6), e83. https://doi.org/10.37175/stemedicine.v2i6.83
Section
Research articles